| dc.contributor | null | spa |
| dc.contributor.author | Cuéllar Ávila, Adriana; Grupo de Inmunobiología y Biología Celular, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá | |
| dc.contributor.author | Cifuentes Rojas, Catherine; Grupo de Inmunobiología y Biología Celular, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá | |
| dc.contributor.author | González Escobar, John Mario; Grupo de Inmunobiología y Biología Celular, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Department of Neurology, Keck Medical School, University of Southern California,
Los Angeles, CA, USA | |
| dc.date.accessioned | 2018-02-24T15:59:00Z | |
| dc.date.accessioned | 2020-04-15T18:09:35Z | |
| dc.date.accessioned | 2023-05-10T17:13:06Z | |
| dc.date.available | 2018-02-24T15:59:00Z | |
| dc.date.available | 2020-04-15T18:09:35Z | |
| dc.date.available | 2023-05-10T17:13:06Z | |
| dc.date.created | 2004-03-10 | |
| dc.identifier | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5035 | |
| dc.identifier.issn | 2027-1352 | |
| dc.identifier.issn | 0122-7483 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12032/91303 | |
| dc.description.abstract | Dendritic cell activity is an important step to elicit cellular immunity. These cells are the most potent antigen presenting cells and the main activating factor for naive T cells. There are several phenotypes of dendritic cells originated from different cell precursors. The aim of this study was to assess a model to obtain human myeloid dendritic cells from peripheral blood CD14+ monocytes. Cells were cultured in the presence of granulocyte-monocyte colonies stimulating factor (GM-CSF) and IL-4 to obtain immature dendritic cells, wich were further cultured with PGE2 plus TNFa to induce their maturation. We obtained immature dendritic cells at day 5 and mature dendritic cells at day 7, according to microscopic cellularphenotype and positivity of membrane markers for CD14, CD86, HLA-DR and CD83, | spa |
| dc.format | PDF | spa |
| dc.format.mimetype | application/pdf | spa |
| dc.language.iso | eng | |
| dc.publisher | Pontificia Universidad Javeriana | eng |
| dc.relation.uri | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5035/3885 | |
| dc.subject | null | spa |
| dc.subject | cytokines, dendritic cells, monocytes, GM-CSF, IL-4. | spa |
| dc.subject | null | spa |
| dc.title | GENERACIÓN in vitro DE CÉLULAS DENDRÍTICAS HUMANAS DE ORIGEN MIELOIDE | spa |
