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dc.contributorOficina de Investigaciones, Universidad Jorge TAdeo Lozanospa
dc.contributor.authorLancheros-Piliego, Daniel
dc.contributor.authorHernández Fernández, Javier; Universidad Jorge Tadeo Lozano
dc.date.accessioned2018-02-24T15:58:18Z
dc.date.accessioned2020-04-15T18:09:15Z
dc.date.accessioned2023-05-11T16:00:25Z
dc.date.available2018-02-24T15:58:18Z
dc.date.available2020-04-15T18:09:15Z
dc.date.available2023-05-11T16:00:25Z
dc.date.created2013-11-22
dc.identifierhttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082
dc.identifier10.11144/Javeriana.SC18-3.apit
dc.identifier.issn2027-1352
dc.identifier.issn0122-7483
dc.identifier.urihttps://hdl.handle.net/20.500.12032/102272
dc.description.abstractWe molecularly identified the loggerhead turtle Caretta caretta using high-speed PCR amplification and restriction analysis of mitochondrial DNA. We isolated the DNA from blood from juvenile C. caretta from Don Diego beach (Magdalena; n=4), in Islas Del Rosario (Bolívar; n=2) in the Colombian Caribbean. By using high-speed PCR amplification of mitochondrial cytochrome c oxidase I (COI), we reduced reaction by one third and obtained fragments of 650 base pairs. We analyzed the amplified IOC product using enzymes HindIII, HpyCH4III and MseI and generated an electrophoretic profile, which compared in silico to other sea turtle species sequences, revealed the loggerhead’s specific pattern. We found similarity between 97-99% with C. caretta in five of the BLAST analyzed nucleotide sequences and 92% in another. We generated a bar code for the sampled turtle information and sequences and stored them in the BOLD database. The methodology described for the identification of C. caretta is a fast and inexpensive procedure that minimizes time and improves PCR specificity.spa
dc.formatPDFspa
dc.format.mimetypeapplication/pdfspa
dc.format.mimetypetext/htmlspa
dc.language.isoeng
dc.publisherPontificia Universidad Javerianaeng
dc.relation.urihttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082/5486
dc.relation.urihttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082/6509
dc.subjectBiología Molecular; Taxonomía; biodiversidadspa
dc.subjectCaretta caretta; High-speed PCR; Cytochrome c oxidase I (COI); AMDAR; Barcode.spa
dc.subjectTortuga cabezona Caretta carettaspa
dc.titleAMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)spa


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