https://hdl.handle.net/20.500.12032/21 2026-04-01T11:34:44Z 2026-04-01T11:34:44Z https://hdl.handle.net/20.500.12032/86144 2023-04-10T17:50:29Z

Attitude scale about professional ethics: a study of higher education teachers in Chile The objective of this research was to report the results of the application of the Attitude Scale about Professional Ethics, proposed by Hirsch, to a sample of higher education teachers in Chile and, based on this report, to perform an exploratory factor analysis to empirically verify the theoretical competencies that are part of this questionnaire. The research results showed that the scale presented high reliability, with a Cronbach's alpha value equal to 0.87. However, the exploratory factor analysis showed that the sample did not recognize the four theoretical competencies of the instrument. When analyzing each dimension separately, by means of factor analysis and reduction to one factor, the questions of the scale with the highest charges were identified. Among the research conclusions, it is suggested for future work to plan an experimental situation to adapt the scale from the theoretical construction, supported by an exploratory factor analysis.; Fil: Gaete Peralta, Claudio Andrés. Universidad Bernardo O´Higgins. Departamento de Matemáticas y Ciencias de la Ingeniería; Chile; Fil: Tapia Sobarzo, Juan Esteban. Universidad Católica de Córdoba. Facultad de Educación; Argentina; Fil: Puraivan, Eduardo H. Universidad Viña del Mar. Escuela de Educación; Chile

https://hdl.handle.net/20.500.12032/86139 2023-04-10T17:50:29Z

Longitudinal Evolution of the Pseudomonas-Derived Cephalosporinase (PDC) Structure and Activity in a Cystic Fibrosis Patient Treated with β-Lactams Traditional studies on the evolution of antibiotic resistance development use approaches that can range from laboratory-based experimental studies, to epidemiological surveillance, to sequencing of clinical isolates. However, evolutionary trajectories also depend on the environment in which selection takes place, compelling the need to more deeply investigate the impact of environmental complexities and their dynamics over time. Herein, we explored the within-patient adaptive long-term evolution of a Pseudomonas aeruginosa hypermutator lineage in the airways of a cystic fibrosis (CF) patient by performing a chronological tracking of mutations that occurred in different subpopulations; our results demonstrated parallel evolution events in the chromosomally encoded class C b-lactamase (blaPDC). These multiple mutations within blaPDC shaped diverse coexisting alleles, whose frequency dynamics responded to the changing antibiotic selective pressures for more than 26 years of chronic infection. Importantly, the combination of the cumulative mutations in blaPDC provided structural and functional protein changes that resulted in a continuous enhancement of its catalytic efficiency and high level of cephalosporin resistance. This evolution was linked to the persistent treatment with ceftazidime, which we demonstrated selected for variants with robust catalytic activity against this expanded-spectrum cephalosporin. A “gain of function” of collateral resistance toward ceftolozane, a more recently introduced cephalosporin that was not prescribed to this patient, was also observed, and the biochemical basis of this cross-resistance phenomenon was elucidated. This work unveils the evolutionary trajectories paved by bacteria toward a multidrug-resistant phenotype, driven by decades of antibiotic treatment in the natural CF environmental setting.; Fil: Colque, Claudia A. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina; Fil: Albarracín Orio, Andrea Georgina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; Argentina; Fil: Tomatis, Pablo Emiliano. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina; Fil: Dotta, Gina. Universidad Nacional de Rosario. Instituto de Biología Molecular; Argentina; Fil: Moreno, Diego M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina; Fil: Hedemann, Laura Gabriela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina; Fil: Hickman, Rachel. Technical University of Denmark; Dinamarca; Fil: Madsen Sommer, Lea Mette. Technical University of Denmark; Dinamarca; Fil: Feliziani, Sofía. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina; Fil: Moyano, Alejandro José. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina; Fil: Bonomo, Robert. Case Western Reserve University; Estados Unidos; Fil: Johansen, Helle Krogh. University of Copenhagen; Dinamarca; Fil: Molin, Søren. Technical University of Denmark; Dinamarca; Fil: Vila, Alejandro. niversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina; Fil: Smania, Andrea M. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina

https://hdl.handle.net/20.500.12032/86142 2023-04-10T17:50:29Z

Utilización del modelo de laboratorio extendido simplificado en secuencias de enseñanza aprendizaje en el ciclo básico del nivel secundario de Córdoba El formato curricular laboratorio, es una de las tantas alternativas para desarrollar Secuencias de Enseñanza Aprendizaje (SEA) en espacios curriculares del Área de las Ciencias Naturales que permite a los docentes propiciar escenarios complejos para que los estudiantes lleven a cabo experiencias significativas de aprendizaje, indispensables para vivenciar lo macroscópico dentro de los niveles de comprensión en los modelos químicos. Este modelo, posible de ser usado en cualquier nivel educativo, es útil particularmente en el Ciclo Básico de la Educación Secundaria donde ocurren las primeras aproximaciones a los tres niveles de comprensión de la Química. Su aplicación, puede sustituir la carencia de infraestructura, materiales y/o falta de adecuación a las normas de seguridad y otras falencias o carencias. El proyecto que aquí se propone, se realizó considerando las problemáticas que presentan algunas instituciones educativas particularmente de la Ciudad de Córdoba para la enseñanza de las ciencias naturales en general y la química en particular. Se trata de un proyecto que toma el Modelo de Laboratorio Extendido en una versión simplificada la cual se adecua a las particularidades de las escuelas secundarias. Esta simplificación permite dar solución a la problemática ya mencionada y también a otras asociadas a la planificación, desarrollo, y evaluación curricular y al uso del laboratorio. Se trata de involucrar en el diseño de las secuencias métodos de innovación, que integren y diversifiquen las estrategias de enseñanza aprendizaje. Y que también utilicen las Tecnologías de la Información y la Comunicación en torno a la alfabetización digita...; Fil: Francia, Romina del Valle. Universidad Católica de Córdoba. Facultad de Educación; Argentina

https://hdl.handle.net/20.500.12032/86140 2023-04-10T17:50:29Z

Common but differentiated responsibilities regarding climate change: different interpretations within the brazilian national context The following paper aims to reconstruct the evolution of this dynamic by tracing the interpretations and reinterpretations of the norm done by Brazilian state actors through the process of localization of the norm in the domestic discourse on climate change in Brazil from 2005 to 2010. The theoretical perspective is based on the literature about norms internalization that attempts to specify the conditions under which international norms find salience in particular domestic contexts. Two main interpretations coexist within the Brazilian political arena in the period within the entry into force of the Kyoto Protocol until the signature of the Paris agreement that influenced the climate politics in Brazil in that period. The first stand around the norm was a traditional interpretation of the principle defending a position of historical responsibilities that implies that Brazil did not have to take greenhouse gas reduction measures. The second position is a more progressive interpretation of the norm that argues that Brazil, as an emergent country, can and must adopt emissions reductions. The tie between both positions allows us to understand the alleged ups and downs in climate policy in the time frame studied.; Fil: Kiessling, Christopher Kurt. Universidad Católica de Córdoba. Facultad de Ciencia Política y Relaciones Internacionales; Argentina; Fil: Pacheco Alonso, Agustina. Universidad Católica de Córdoba. Facultad de Ciencia Política y Relaciones Internacionales; Argentina